Fig. 3
From: Canine intestinal organoids as a platform for studying MHC class II expression in epithelial cells
IFN-γ treatment of canine colonoids. (A) A schematic representation of the flow of the IFN-γ treatment. The colonoids initially cultured for 2 days in EM, followed by changing the medium from EM to DM. At day 4, the colonoids were treated with 0, 30 and 60 ng/mL of IFN-γ. For comparison, colonoids treated with IFN-γ in EM were used. The colonoids were then collected at 0, 24 and 48 h after IFN-γ treatment for further analysis. This schematic was created with BioRender.com. (B) The representative image of the canine colonoids in DM at day 6. Scale bar = 50 μm