Fig. 4
From: Canine intestinal organoids as a platform for studying MHC class II expression in epithelial cells
MHC class II related gene expression in colonoids after IFN-γ treatment. DLA-DRA and CIITA mRNA expression was assessed using RT-qPCR at 0, 24 and 48 h after IFN-γ treatment (0, 30 and 60 ng/mL) in EM and DM. Three biological replicates were used for this analysis and cytokine treatment was replicated three times. Significant differences between the EM and DM control groups (0 ng/mL) at 24 and 48 h are indicated with bracket-style markers. Significant differences between IFN-γ-treated groups (30 or 60 ng/mL) and their respective EM or DM control groups are indicated with flat line markers. The error bars represent the SEM. **p< 0.01, ***p< 0.001